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Understanding the sequence of events in assembling multi-protein complexes is crucial for comprehending DNA repair processes and identifying potential drug targets. LUMICKS’ dynamic single-molecule approach provides such insights, even within the physiological environment of cellular extracts.
Case study
Professor Ben Van Houten‘s team at the University of Pittsburgh successfully used cellular extracts without complex protein purification to
Furthermore, using the C-Trap’s ability to apply precise mechanical stress on individual molecules, they discovered a correlation between PARP1 binding kinetics to nicks, and the mechanical state of the DNA substrate.
Step 3: Easy-to-use data analysis provides insights into the steps, kinetics, and potential variations of the molecular process.
Step 2: Single-molecule fluorescence imaging allows the identification and real-time tracking of multiple types of proteins on the DNA substrate.
Step 1: Proteins are expressed in cells and nuclear extracts are loaded into the C-Trap where they interact with well-defined DNA substrates.
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Understanding of DNA repair mechanisms could advance treatments for cancer and diseases of aging. But reconstituting DNA repair protein complexes from cancerous tissues to study their mechanisms of action is often time-consuming or, in some cases, impossible. A new technique performing dynamic single-molecule analysis directly on nuclear extracts allows rapid mechanistic analysis of mutant proteins from cancer cells, providing previously unseen insights into their mechanisms of action. This new innovative tool, when combined with rapid data analysis, represents a bridge between the study of biochemistry of purified proteins and molecular biology.
Static structural data often limits hypotheses and models explaining DNA-protein binding mechanisms, failing to capture complex dynamics.
Case study
Ingrid Tessmer (Rudolf Virchow Centre Würzburg) and her team employed the C-Trap to directly visualize DNA translocation and lesion recognition by O6-alkylguanine DNA alkyl-transferase (AGT).
This approach unveiled details of AGT’s capabilities to
Real-time observation with the C-Trap provided quantitative insights, challenging established models of AGT’s unidirectional movement, previously believed to be accelerated by cluster formation.
DNA lesion search dynamics. One-dimensional diffusion constants (D) on DNA plotted over the lifetimes of complexes on the DNA for (A) AGT and (B) ATL. The insets show representative kymographs (green traces) obtained by fluorescence microscopy-coupled dual trap optical tweezers, in which the y direction corresponds to the positions on the DNA tether (shown schematically between two beads held in the two optical traps), and the x direction to time. Images from: Tessmer et al. Int. J. Mol. Sci. 2024
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In this session, hosted by DNA repair expert Dr. Ingrid Tessmer, Rudolf Virchow Center for Experimental Biomedicine, University of Würzburg, we dive deep into the role of alkyltransferase-like proteins (ATLs) and their role in NER. Despite their inherent catalytic inactivity, ATLs play a remarkable role in targeting alkyl lesions for repair by the NER system. Through a combination of single-molecule and ensemble methodologies, a detailed view of the recruitment process of UvrA – the initiating enzyme of prokaryotic NER – to an alkyl lesion by ATL has been observed for the first time.
Moreover, we delve into the mechanisms of lesion recognition by ATL, and illustrate the dynamic DNA lesion search undertaken by highly active ATL and ATL-UvrA complexes.
Don’t miss this opportunity to broaden your understanding of DNA repair and its potential role in revolutionizing cancer treatment strategies.
Preventing DNA end disjunction is vital for DNA double-strand break (DSB) repair, yet the role of PARP1 in this process remains unclear.
Case study
Simon Alberti (MPI-CBG Dresden) and collaborators used LUMICKS’ dynamic single-molecule approach with the C-Trap to investigate PARP1 recruitment to DSBs. Single-molecule imaging, combined with measuring mechanical forces, revealed that
Representative florescence images of mEGFP-tagged PARP1(WT), PARP1(ΔC), and PARP2(WT) localization on tethered λ DNA, which was nicked on both strands by CAS9(D10A) with a 60 bp gap between the nicks. Only PARP1(WT) shows condensation at the damage site. Scale bar, 2 μm. Image source: Chappidi et al. Cell 2024
Force measurement as a function of distance from tethered λ DNA extended in the presence of mEGFP-tagged PARP1(WT) or PARP1(ΔC) or PARP2(WT) (1 μM). Thick line represents the mean value. Image source: Chappidi et al. Cell 2024
Distance measurement as a function of time from force clamped λ DNA or λ DNA harboring a PARP1 condensate. Thick line represents mean value. Image source: Chappidi et al. Cell 2024
Workflow of the optical tweezer force clamp and force-extension experiments. Image source: Chappidi et al. Cell 2024
Homologous recombination (HR) is crucial for repairing double-strand breaks (DSBs) in DNA, yet some molecular mechanisms and protein roles remain unclear.
Case study
"These cellular actions of BCDX2 have been a puzzle for over 20 years."
Utilizing the C-Trap, studies led by Steve West, Simon Boulton (both at The Francis Crick Institute, London), and David Rueda (Imperial College, London) uncovered new sub-steps and regulatory functions in RAD51 filament formation, a key HR process:
These findings elucidate two distinct BRCA2-dependent RAD51 loading mechanisms onto ssDNA and illuminate the roles of recombination mediators during filament growth.
Understanding these processes at the single-molecule level clarifies the function of BCDX2 and other factors in RAD51 assembly on ssDNA, which is crucial for replication fork protection and DSB repair, and essential for tumor avoidance.
A representative kymograph showing diffusion-driven delivery of BRCA2-RAD51 complexes to ssDNA in the vicinity of the ds-ssDNA junction. Static BRCA2-eGFP molecules bound directly to the ssDNA gap. Mobile BRCA2-eGFP molecules diffuse along dsDNA. 25 nM RAD51-A466 (red) was incubated with DNA in the presence of 5 nM BRCA2-eGFP (blue) and 1.25 nM RPA in the presence of 5 nM SYTOX Orange. Position of the ssDNA gap is indicated by dashed lines.
Schematic of RAD51 filament binding experiment, in which λ DNA was pre-incubated with a 1:1 mixture of labeled and unlabeled RAD51 and then moved to a channel containing BRCA2-eGFP to monitor BRCA2 binding.
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DNA repair is a highly complex and dynamic process that involves the interplay of numerous different proteins and components. The helicase HELQ is known to play a role in double-stranded breaks (DSBs) repair, but its molecular mechanisms remain unknown.
A study by the research group led by Simon Boulton presented how dynamic single molecule analysis leads to direct visualization of the mechanism of HELQ in DNA double-stranded breaks (DSBs) repair. Check out this application note to learn more.
Double-stranded breaks (DSBs) in DNA pose significant threats, contributing to cell death and cancer. POLQ, an enzyme implicated in DSB repair, is often overexpressed in cancers, bolstering cancer cell survival.
Case study
Researchers at The Francis Crick Institute, led by Simon Boulton, utilized the single-molecule imaging capacity of the C-Trap to
This discovery aligns with previous findings showing a synergistic effect between POLQ and PARP inhibitors, a crucial therapy for BRCA-deficient cancers. Simon Boulton’s team suggests that POLQ inhibitors could serve as potent second-generation drugs for treating PARP-inhibitor-resistant BRCA-deficient cancers, shedding light on potential novel treatment avenues.
Directly observing catalytic protein activity (e.g. POLQ processing ssDNA) in real-time, the mode of action of small-molecule or other inhibitors becomes directly visible and quantifiable.
The C-Trap® provides the world’s first dynamic single-molecule microscope to allow simultaneous manipulation and visualization of single-molecule interactions in real time.