Study and visualize DNA-binding proteins at the nanoscale
Use Dynamic Single-Molecule to obtain the full understanding of DNA-binding proteins
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Why Dynamic Single-Molecule?
The interactions and dynamics of DNA-binding proteins in action
Today’s scientific trends are racing towards smaller scales and experimentation that provides both structural and mechanistic insights. To decipher biomolecular mechanisms you need methods capable of detecting the interactions between proteins and nucleic acids as they happen and at the molecular level. DNA-binding proteins are key regulators of genome function, guiding essential processes like gene expression, replication, and repair. Yet, bulk assays often miss the dynamic search, binding, and release behaviors that define their activity. Without real-time, single-molecule insights, the true complexity of these interactions remains hidden.
Overcome these challenges with Dynamic Single-Molecule technology through:
- Visualize how DNA-binding proteins locate, bind, and move along DNA at the single-molecule level
- Measure binding kinetics, affinity, and specificity in real time
- Uncover how these proteins regulate processes like transcription, replication, repair, and genome organization
Explore your research application
Explore what Dynamic Single-Molecule can mean for your field of interest
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RNA Translation
Study and visualize RNA translation mechanisms at the nanoscale
Use Dynamic Single-Molecule to obtain the full understanding of translation mechanisms
Available case studies:
Investigation of ribosome activity and states
Case study
Measurement of riboswitch conformational changes
Case study
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DNA Replication
Study and visualize DNA replication mechanisms at the nanoscale
Use Dynamic Single-Molecule to obtain the full understanding of replication mechanisms
Available case studies:
The replication machinery: Identify key players and their diverse roles
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Real-time insights into origin recognition and replisome formation
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Replication in context: Uncover mechanisms ensuring replication fidelity and genome stability
Case study
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DNA Transcription
Study and visualize DNA transcription mechanisms at the nanoscale
Use Dynamic Single-Molecule to obtain the full understanding of transcription mechanisms
Available case studies:
Real-time observation of DNA exonuclease dynamics at base-pair level
Case study
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DNA Repair
Study and visualize DNA replication processes at the nanoscale
Use Dynamic Single-Molecule to obtain the full understanding of repair mechanisms
Available case studies:
Characterize the (dis-)assembly kinetics of repair complexes based on single-molecule real-time data
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Directly observing molecular search and repair mechanisms delivers unexpected insights
Case study
Pulling on individual molecules reveals how biomolecular condensation physically prevents DNA end disjunction
Case study
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DNA Organization
Mechanisms and roles of chromatin organization – decipher the epigenetic code
Available case studies:
Quantify SMC activity, conformation and interactions at the molecular level
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Follow chromatin remodeler activity in real-time
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Use the force: Quantify nucleosome stability and cross-linking
Case study
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DNA/RNA Structure
Reveal the structural dynamics of RNA & DNA in real time
Use Dynamic Single-Molecule to obtain the full understanding of DNA/RNA structure
Available case studies:
Uncover structural dynamics in telomeric RNA for cancer research
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Investigate RNA misfolding in neurodegenerative disorders
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Reveal protein-RNA interactions critical for viral replication
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DNA Editing
Study and visualize DNA editing mechanisms on the nanoscale
Use Dynamic Single-Molecule to obtain the full understanding of editing mechanisms
Available case studies:
Understand off-target activities of Cas for safer gene editing
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Real-time insights into gene editing mechanisms
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Solutions
C-Trap
Biomolecular interactions re-imagined
The C-Trap® provides the world’s first dynamic single-molecule microscope to allow simultaneous manipulation and visualization of single-molecule interactions in real time.
Technical note:
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This shows the most recent card of each resource type filtered on Business Unit
Webinar, Scientific update, Whitepaper, Application note, Brochure.
We only show 4 and we have 6 types so the 2 older ones are hidden.
In design only 1 is shown, but the rest will be loaded when published.
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New Insights in Chromosome Organization: Single Molecule Analysis of Eukaryotic SMC Complexes
Webinar
March 31, 2025
01-01-20
Structural Maintenance of Chromosome (SMC) complexes, cohesin, condensin and Smc5/6, play a fundamental role in genome organization, facilitating chromosome compaction, segregation, and DNA repair. Despite their essential functions, the mechanisms by which the complexes interact with different DNA substrates and influence topological transitions remain not fully understood. Using the LUMICKS C-Trap, we have employed single-molecule approaches to analyze the behavior of purified SMC complexes, focusing on yeast cohesin and human SMC5/6, on different DNA substrates, including double-stranded (dsDNA) and single-stranded DNA (ssDNA). Additionally, we have used a quadrupole optical trap to bridging by SMC complexes and their effect on DNA decatenation by Topoisomerase IIα (Top2A). These findings provide new insights into the fundamental properties and requirements of cohesin and Smc5/6’s interaction with DNA substrates, as well as their ability to bridge two independent DNAs.
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Deciphering the Dynamic Mechanisms of Thymine DNA Glycosylase (TDG) in DNA Repair
Scientific update
December 5, 2024
01-01-20
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Golden Gate meets C-Trap: A powerful combination for unprecedented molecular insights
Application note
December 18, 2024
01-01-20
Precisely manipulating genetic material at the single molecule level is gaining importance across life sciences – and so do the tools that allow researchers to do exactly that. The C-Trap system combines single molecule fluorescence microscopy with optical tweezers to manipulate DNA, allowing researchers to directly observe and track molecular events as they occur. Designing and creating specific DNA constructs is crucial for maximizing the potential of single molecule studies. In this application note we introduce the powerful combination of cutting edge biochemistry and single-molecule visualization methods to increase throughput and maximize the results gained from each individual measurement.
C-Trap Product Brochure
Brochure
February 28, 2025
01-01-20
AACR 2025
Conference
April 18, 2025
01-01-20
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