Avidion
True binding. Better therapies.
Ideal for cell therapy candidate screening and large characterization studies. Run up to 4 disposable 48-well cartridges a day for a total of 192 measurements with <80 min. hands-on time.
High throughput
High throughput measurements with 96-well plate compatibility
Hands-off
Minimal hands-on time through automated handling and deep-learning analysis
Multiplexed
Multiplexed insights through 4-color fluorescence readouts
Introduction video
Prepare for cellular lift-off
Take a look at our introduction video to learn everything about Avidion
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Workflow
Easy to use, impactful results
Measure the overall strength of interaction between cells or between proteins and cells, quickly and easily.
Introduce your target cells using standard well plates
Load up to 4 cartridges of target cells, adherent or non-adherent, using standard 96-well plates. Avidion automatically transfers the cells and ensures proper incubation through various checks.
Add your media and effector cells, up to 192 per day.
Introduce up to 192 fluorescently labeled effector cells in separate 96-well plates. Avidion automatically introduces them to your target cells, handling incubation and timing.
Let Avidion execute your measurements
Let the Cell Avidity measurement begin. Through a sequence of multicolor fluorescence imaging and centrifugal force application, true cell binding is revealed.
View your results and start planning for tomorrow.
Get direct access to Cell Avidity insights thanks to Avidion's powerful AI-accelerated on-board analysis. With disposable cartridges and automated self-cleaning, you can shift your attention to your next experiment.
Avidion in the lab
Experience how easy it is to adopt our Avidion solution in your own lab. We'll take you through the necessary preparation and experimental execution steps.
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Multiplexing features
Multiplex your colors, Multiply your insights.
Staining with small molecules
Unlock deeper insights with signal-specific gating
Activation and viability markers can be used to add functional context to your studies as well as achieve excellent data quality.
Figures: Jurkat cells on Nalm6 were kept too long in culture medium to create artificially high concentrations of dead cells. Apoptotic and necrotic cells were labelled with Caspase and Sytox, respectively. The bar plot shows that gating apoptotic and necrotic cells enhances the Cell Avidity measurement, as would be expected.
Staining of surface receptors with antibodies
Differentiate subpopulations with precision
Measuring Cell Avidity across phenotypes is made possible through characterizing subpopulations like CD4/CD8 T cells or memory versus effector states.
Figures: Purified CD4+ and CD8+ cells were mixed and expanded in culture for 14 days. CD8 antibody PerCP-Cyanine5.5 and CD4 antibody FITC were used together with Cell Trace dye. CD4+ and CD8+ cells were gated and the bar plot shows cell avidity for each subpopulation.
Detection of fluorescent proteins
Streamline transduction analysis without extra staining
Fluorescent protein expression can be leveraged in order to assess CAR levels and co-expression markers.
Figures: Endogenous expression of GFP was measured in CD8 primary T cells.
Throughput
Screen 192 samples within 80 min. hands-on time
800 cells
Up to 800 cells analyzed per well
4 colors
Cell Avidity + 3-color fluorescence for multiplexed insights
48-well cartridge
Up to 48 wells per Avidion cartridge
192 samples per day
Run up to 4 cartridges per experiment within 80 min. hands-on time
Case study
How many Cell Avidity measurements can you do in a day? A good example is a CAR-T cell screening project performed in collaboration with Professor Dirk Busch at Technical University Munich, using Avidion. In just half a day, it is possible to test and rank 20 CAR constructs.
Rapid screening
20 Jurkat CAR constructs screened in a single day. Each sample was measured with 4 technical replicates.
Cost and time efficiency
Early, robust identification of top candidates
Repeatable and reproducible results
Tight error bars and SDs confirm assay robustness.
Customer testimonials
How Cell Avidity empowers pioneers of immuno-oncology
Mark is a global pioneer in translational cell and gene therapies
"Our study identified cell avidity as a key predictor of in vitro function, offering a pathway for rapidly developing more effective NK cell-based immunotherapies. Cell Avidity measurements [...] provide key information that can accelerate immunotherapy development by accurately predicting in vivo and clinical efficacy."
Mark Lowdell, PhD
Chief Scientific Officer
Inmune Bio
Marcela develops CAR T cell therapies to treat cancer
"Interestingly, of the pre-clinical assays we used to compare CAR constructs, only [Cell] Avidity correlated with in vivo results."
Marcela Maus, MD, PhD
Director of Cellular Immunotherapy
Massachusetts General Hospital
Peter's team explores how to create better cell-based therapies
"[Tuning Cell Avidity is] a novel method for synaptic enhancement of CAR-NKs, [which] leads to increased cellular avidity, improved function, tumor clearance and survival in in vivo solid tumor models. There’s something very elegant about the solution. Avidion makes it exceptionally straightforward."
Peter Chockley, PhD
Assistant Professor
The Ohio State University College of Medicine
Lydia's team studies how immune system dysfunction drives myeloma progression and develops rational immunotherapeutic strategies
"In our exploration of the reasons underpinning suboptimal clinical responses in the AUTO2 trial, Cell Avidity allowed us to establish poor tumor binding (avidity) by cell-bound APRIL compared to competitor CARs as a likely explanation."
Lydia Lee, PhD
Clinical Senior Research Fellow
University College London
Platform
Take a look inside
Discover the advanced technology and engineering that makes Avidion possible
Robotic cartridge handling
Seamlessly moves your cells through all of the Avidion's compartments, delivering near hands-free operation.
Liquid handling system
Automatically manages media exchanges and reagent additions, ensuring consistent and reproducible biology.
Deep-learning enabled graphical processing
Delivers exceptional data quality through automated, intelligent quantification, ready for you right after your experiment.
Centrifuge
Gently applies a controlled force to assess multi-receptor binding, distinguishing strong interactions from weaker ones.
Multicolor fluorescence imaging
Unlocks multiplex imaging and enables unified experiments including cell population phenotyping, receptor expression, and transduction efficiency.
Incubator
Ensures optimal conditions for your target cells in preparation for your experiment.
Software
Your new data acquisition and analysis experience
Design, execute and analyze your experiment with ease and confidence. Our software delivers an intuitive experience combined with powerful algorithms for the most complex binding behavior.
Design and prepare your experiment with ease
Design the perfect experiment with our intuitive interface. You’ll receive a lear overview of experiment phases, reagents and well plate layouts.
Enjoy an automated workflow with minimal hands-on time
Make use of our guided experience with clear actions and information presented throughout your whole experiment.
Obtain immediate insights and study them wherever, whenever
Your data is ready straight after the experiment. Study and analyze it from the comfort of your desk, or home.
Key content
Download the brochure
Get the latest all-in-one overview
Avidion Product Brochure
Brochure
The latest all-in-one overview of Avidion’s features, options, and specifications.
Benefits
Beyond the product
Take a look at the perks that come with being one of our users
Symposia
Join the community
BIT (Breakthroughs in Immunology) is LUMICKS’ symposia format. We regularly host events across the world, aiming to bring together scientists interested in Cell Avidity to share their research, connect, and exchange ideas and experiences.
Support & service
Your success is our success
Our goal is to minimize your time to results, ensure success, and provide instruments that are easy to use and maintain. To this end we have dedicated teams providing user training, application support and system check-ups.
Adopting Cell Avidity
Let’s begin your LUMICKS journey
Our experts are ready to learn about your research challenges and see where our technologies can bring value.
Demonstrating value
Our application scientists can help create interest among potential users through organizing different events such as seminars, workshops, and demonstrations, as well as meet with stakeholders individually to understand and help solve their needs.
Avidigo services
At our Amsterdam headquarters, our internal Avidigo service team supports academic and industry clients looking to integrate Cell Avidity into their workflows and pipeline. From experimental design to data analysis and interpretation, we support you all the way.
Grant & tender support
Throughout our history we have supported multiple successful grants across a broad spectrum of funding and users involved. Our application scientists are experienced in highlighting the unique value of Cell Avidity and its solutions.