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This step-by-step guide is intended as a reminder of what was covered during the full LUMICKS C-Trap training, and not as a stand-alone detailed operating protocol. This guide specifically addresses assays using the TIRF (i.e. oil) objective, with widefield, TIRF or IRM imaging. A near surface DNA-tether assay or a surface-bound microtubule assay are both typical examples of such assays. Differences may exist between systems and may not be captured here.

This step-by-step guide is intended as a reminder of what was covered during the full LUMICKS C-Trap training, and not as a stand-alone detailed operating protocol. This guide specifically addresses assays in solution (far from surface) such as DNA dumbbell experiments using the LUMICKS flowcell.

This article outlines the steps to take when uneven flow (or clogging) and/or bubbles are observed when using the LUMICKS microfluidics system and flow cell

This guide outlines all the steps from sample preparation to data analysis for typical cell membrane interaction experiments on the C-Trap. This document starts as a start point for you to define your own experimental conditions and workflows

In this tutorial, you can find the Pylake installation instructions and a few simple examples to get started in Pylake.

Protein unfolding experiments from sample preparation to data analysis