Video tutorial

Making and mounting a custom flow chamber

Abstract

This short video shows how to assemble an example custom chamber for cell experiments on the C-Trap. Adherent cells are seeded and cultured on glass coverslips. A coverslip is then mounted on a glass slide with double-sided tape, and the chamber is subsequently sealed with vacuum grease. The assembled custom chamber can be mounted on a LUMICKS custom flowcell holder and placed on top of the objective.