Molecular determinants of Smc5/6 association with DNA junctions

Smc5/6 is an essential genome maintenance complex that regulates DNA replication and repair.  Its roles in managing DNA intermediates generated from these processes are intimately linked to its ability to associate with various forms of DNA. To gain insights into how Smc5/6 engages with different DNA structures, we use the C-Trap single-molecule setup in combination with several DNA substrates. We will presence our recent findings of how Smc5/6 preferentially associates with DNA junctions flanking a ssDNA gap, a common DNA intermediate generated during genome maintenance processes.  We will also discuss how other genome protecting factors can influence Smc5/6 junction binding behaviors and the implications of our findings in genome maintenance processes.

What you will learn in this webinar:

• Watch how Smc5/6 preferentially associates with DNA junctions flanking ssDNA gaps.
• How C-Trap data reveals binding behaviors that are lost in traditional bulk assays.
• How genome-protecting factors dynamically influence Smc5/6's path to the site of damage.
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